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The abstracts and news articles were then placed and classifed in chronological order in an Excel table in accordance with the research setting or institution concerned as recorded in the news item (for the dailies) or to which the primary author was linked (for Medline) (grouping carried out in accordance with emerging institutional categories) nitroglycerin 6.5 mg line medicine list. Each abstract or press item represented an event generic nitroglycerin 2.5mg line symptoms 9dp5dt, that is, research results (especially for the scientifc feld) or information that marked the pathway of the drug. Thus all the scientifc abstracts reporting results of studies of the medication or substance in question were included in the analyses, but only news items with information that could impact on the future of the drug were considered (by consensus of the analysts) to the exclusion of individual comments. It may go from point 1 to point 6 by looping around within the rooms or just as easily move in a single loop from point 1 to point 6. Point 6 is the computer room and is used to look for scholarly articles on the Internet and to refne the experimentation projects. Different technical aspects are involved in these instruments and bring into play specialized knowledge from outside the realm of pure biology, such as physics, chemistry and information technology. The laboratories also include offces and meeting rooms that are used at all stages of research. The facilities of the northern, regional-university laboratory differ signifcantly from those of the frst two. Much of the activity in this unit involves the description of compounds of essential oils and plant extracts from the boreal forest, so the role of the isolation, extraction and purifcation rooms is crucial. Once the extracts are prepared or the fnal products isolated and purifed, they can be sent on to the biology laboratories where biological activity tests can be conducted. On the biology side, a microbiology room and a tissue culture room are available, but they are separated from each other. These two rooms connect with a central room where the devices and computers for collecting and analyzing data are located. A molecular biology room also adjoins the laboratory so that research can be pursued further and the mechanism of action of the biopharmaceutical agents isolated by the chemists can be elucidated. Animal quarters are also available for in vivo research in a room located just next to the biology laboratories. Close cooperation between the chemistry and biology laboratories is therefore vital, even if they are physically separate. As in the other two laboratories, all these rooms converge on the computer room, indicating the importance of the circulation of knowledge, whether from the outside in or vice versa. The premises and the equipment installed there are apparently not completely standardized, although certain principles in the use of the instruments limit fexibility. They thus refect a degree of diversity stemming from the complexity of different intervening levels. As the examination of these organizations shows, instrumentation affects strategic choices: either a laboratory maximizes its return from the technologies at its disposal, or it tries to acquire the instrumentation to meet its research objectives, in which case the political/fnancial and technical/clinical levels intervene, levels that bring into play concepts representing different disciplines. In fact, the number of pathways increases in accordance with the instrumentation used, the complexity and/or novelty of the substance under study and the unpredictability of its effects. But a more important aspect is the movement back and forth between the different levels. In addition, the knowledge derived from all the operations is not the product of the observation of a direct effect, for it is not possible to observe the behaviour of cells or proteins directly under a microscope. Indeed, staining and coupling with light-emitting particles are used to provide indicators of the effect under study because they are the only way of verifying cell behaviour. Knowledge may therefore follow variable pathways, particularly circular ones regulated by a systemic mode of organization. Through the relationships that emerge from its physical organization, the laboratory delivers the means by which the knowledge wanted and needed to accomplish its mission may be appropriated and may operate. The networks of actors The networks of actors associated with publications produced by the laboratories were reconstructed for the entire scientifc career of the director of the two related Montreal- area laboratories.
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